Affid grafting using autologous tissue (AFG) is an method that has been in use for quite a while. When Dr. Neuber carried out oncological surgery in 1893 Neuber was among the first doctors to employ fat grafting for treating injuries and complications, such as deformities and wounds. Since their initial use natural autologous fillers have experienced growth in both their uses and procedures.

Both in reconstructive and cosmetic procedures, fat grafting has become more commonplace in the past 20 years. The enlargement of the breast and buttocks facial atrophy and rejuvenation scar contractures, hand rejuvenation are only some of the numerous reasons to use the autologous fat-grafting (AFG).

It is possible to correct form and other issues by autologous fat grafting an aesthetic and reconstructive method that involves the patient’s fat is removed and injected into soft tissues. Biocompatibility, lack of immunogenicity and the access to fat grafts make it the best filler for soft tissues. Aeftkraaftake and effectiveness are affected by the entire fat grafting procedure from collection through processing, and finally transplantation. In this article we will look at the most commonly used methods to process fat grafts like centrifugation, rolling gauze, sedimentation and washing and filtration.

The process of fat graft (แฟตกราฟ, which is the term in Thai) processing has the greatest importance because of its sensitivity to Adipocytes, since blood and other undesirable cells are removed. There isn’t a conclusive proof of whether one particular fat-graft technique is superior to another as there is a shortage of research that is well-defined and compared the various methods.

A Look At The Research Behind Fat Grafting

Once it has been retrieved, fat is processed which eliminates any blood, fluid cell fragments, cell fragments, or oil. Once these contaminants are gone then the remaining fat is well-prepared for future injections and fat graft. Many different kinds of cells such as endothelial cell, predipocytes, endothelial progenitor cell lines and fibroblasts, vascular muscles, as well as, most importantly adipose-derived stem/stromal and stromal cells are present in an isolated fat grafts, also called a stromal-vascular fraction (ASCs). Mesenchymal stromal/stem cells among which ASCs constitute a distinct subset are multipotent and can grow into a variety of types of cells which include but not restricted to adipocytes osteoblasts, chondrocytes, as well as myocytes.

It is thought that ASCs increase the fat graft taking by helping to improve the revascularization process of the fat that has been transplanted. After the transplant the fat graft may be separated in three different regions.

• The regenerative zone the place where ASCs replace dying adipocytes.
• The necrotic zone in which both ASCs and adipocytes have gone out of existence and have been transformed by connective tissues or scartissue.
• The avascular zone in which ASCs as well as connective tissues have replaced Adipocytes.

Adipose tissue transplants therefore have a dynamic changing process that is enhanced by ASCs. After a fat graft been harvested, it needs to undergo processing in a manner which preserves as much of the adipocytes as well as ASCs as is possible. Separation by gravity (sometimes called sedimentation or decanting) centrifugation, gauze rolling, as well as the washing and filtration system are a few of the present methods for fat processing following the harvesting of fat.

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